This study evaluated the effects of massage therapy on immune functions of two healthy females. Using a single-case experimental ABAB design, two subjects received a relaxing massage during the experimental phases (B) and no massage during baseline phases (A). Assays were conducted for T and B lymphocyte mitogen-induced proliferation, enumeration of T and B lymphocyte subsets, and quantification of IgA, IgG, IgM and cortisol levels. Trait and state anxiety levels were also examined. The results indicated a consistent and significant trend of increased activity of both T and B lymphocytes and levels of serum IgG for both subjects during the B phases compared to the A phases. There were no other significant differences between experimental and control conditions for the remaining measures, although serum IgM levels approached significance. Both subjects demonstrated a reduction in trait anxiety over the period of massage therapy.