The objective of this study was to evaluate whether energy medicine (EM) practitioners can perceive the presence of cultured human cells without visual cues. Three randomised, double-blinded pilot studies were used to evaluate the ability of participants to distinguish a flask containing cancer cells growing in culture medium from a flask containing either culture medium or sterile water. Each study consisted of six independent experiments: three with EM practitioners and three with non-practitioners. The number of independent trials for each experiment was estimated by the statistical power analyses of the design. Practitioners’ feedback from the first two studies was used to revise the protocol for the subsequent studies, with the intent to eliminate potential problems in making this distinction. Labelled flasks (‘cells’ and ‘no-cells’) were added to serve as references for comparison in the second study and the number of experimental trials was reduced in the third study. Eight experienced EM practitioners and nine non-practitioners (laboratory personnel with no EM training) participated. In the first two studies, the number of correct determinations in a series of 34 trials was recorded. In the third study, the number of correct determinations in a series of 10 trials was assessed. All participants performed at the level expected by chance.