The aim of this RCT was to investigate the effects of music on the anxiety of mechanically ventilated patients, as assessed by objective parameters and a subjective validated anxiety scale. A total of 64 subjects was randomly assigned to undergo either 30 min of music intervention or a rest period. The subjects were asked to answer the STAI scale before and after the study period and physiological indices and resting behaviours were recorded before and after the study period in both groups. The subjects’ satisfaction with music was also obtained after music intervention. Patients who listened to a single 30-min session of music appeared to show greater relaxation as manifested by a decrease in physiological indices and an increase in comfortable resting behaviours.